Introduction:
여러 질병의 현상을 통합적으로 설명할 수 있다면치료하는데 있어서 유익한 결과를 가져올 것이다.
흡연, 고혈압, 당뇨, 비만인 사람들에게서 TGF-β수치가 증가함
Objective: 여러 질병들에 대한 TGF-β의 신호전달에 대한 역할에 대하여 알아보고자 함
TGF-β는 조직의 섬유화, 상처, 흡연,
et al. 2005).
Enhanced the immune functions in cell studies(Blondin et al. 1996;Hsu et al. 2001).
However, the effect of fucoidan effect on adipocyte development has not been reported in the literature.
Obesity and other diseases such as coronary artery disease are closely associated with adipocyte differentiation and subsequent extent of fat accumulation(kawada et al. 2001; Sharma 2002).
Mid-1800s - Beginning research of Embryo
1908 – ‘Stem Cell’ from Alexander Maksimov
1963 – Self reproducing cell
1978 – Found Blood stem cell
1981 - Embryonic stem cell
1998 - Human's embryonic stem cell
2001 – First human embryo cloning
(중략)
2.1 Adult Stem Cell - How?From Bone Marrow
HSCs and MSCs are rich in b
Ⅰ. 서 론
크레아틴인산효소(Creatine Phosphokinase ; CPK)는 전이효소로서 인산기 전이 작용을 하며, 근수축시 근유내 ATP농도의 일정한 유지를 위한 인산 동원에 이용한다. 젖산탈수소효소(Lactate Dehydrogenase; LDH)는 산화환언 효소로서 탈수소효소로서 해당과정에서 초성포도산을 환언하여 젖산을 생성한다(Mur
Enamel
√ Hard and white substance covering the teeth
√ The hardest and most highly mineralized
√ Protects the fragile underlying parts of the teeth
√ Covers the crown and supported by dentin
Component of enamel
√ 96% of enamel : mineral, water, organic material
√ contain two unique classes of proteins amelogenins and enamelins
√ The basic unit of enamel is called
Stem cell calssification
1. Based upon location
Embryonic stem cells
Somatic/ adult stem cells
2. Based upon function
Totipotent
Pluripotent
Multipotent
Unipotent
Reject Response
Traditional Immuno-repressents
Constant administration
Little engraftment after 3~4 weeks
Host immune system impaired
Vulnerable to Infection
↓Need brief immuno-re
1. 이번 실험을 통해 세포의 freeze, thaw, subculture, maintain 하는 방법에 대해 알아보고자 했다. 이 때 각각의 단계에서 어떠한 실험 과정을 거치는지에 대해 이해하는 것이 목적이다.
2. 이 때 dish 위에 깔린 cell의 수를 재는 것도 중요한데 이러한 cell counting을 하는 방법을 아는 것이 또 다른 목적이다. cell c
Histologically, the islets consist of three major and at least two minor cell types.
Beta cells, which synthesize and secrete insulin, make up about 60% of a typical islet.
Alpha cells are the source of glucagon, 30% of islet tissue.
Delta cell, which are less abundant, produce somatostatin.
F cells, which secrete pancreatic polypeptide.
Fifth cell type that secretes ghrelin.
Fifty cell typ
A. Limitations
So far we have seen a new approach of using stem cells for targeting cancer site and a new concept of
treatment. However it might seem fascinating, it is important to note that significant impediments remain
before the true potential of this novel therapy can be fully realized. Here in this section we are going to
mainly focus on the limitations of neural stem cells esp
5. CrCl3에 의한 Insulin 분비능
11 mM glucose를 처리한 군에서 insulin secretion은 25 mM glucose 자극에 대해 131% 증가했다. 33 mM glucose를 처리한 군에서는 동일한 자극에 대해 20 %의 증가를 보였고 CrCl3를 첨가한 군에서는 6 %의 증가치를 보였다.
Fig. 5. 33 mM-induced cytotoxicity in INS-1 cells. INS-1 cells exposed to various concent